ACEGID Trains Rwandan Scientists in Next Generation Sequencing of SARS-CoV-2

ACEGID facilitated a three-week training on SARS-CoV-2 sequencing and bioinformatics for Rwandan scientists at the Rwanda Biomedical Centre (RBC).

The training was held as a collaboration between ACEGID and RBC from January 31 to February 18, 2022.

ACEGID scientists trained participants in the principles and practices of SARS-CoV-2 sequencing steps including sample handling, extraction, amplification, quantification, library preparation and sequencing. The participants had hands-on training on Illumina sequencing with Miseq and Oxford Nanopore Technology (ONT) MinION.

Participants were also trained in bioinformatics. They learnt how to assemble sequences, how to submit data to online repositories, how to identify variants and how to interpret data.

Twenty-two scientists from three Rwandan public health institutions were trained in the workshop. 122 SARS-CoV-2 genome sequences (50 on ONT MinION and 72 on Illumina Miseq) were assembled during the training.

Participants’ feedback showed enthusiasm for applying the lessons learnt towards fighting infectious diseases in Rwanda. Below are excerpts:

Frankly speaking, I don't have enough words to express my gratitude for what you did for us. You took us from almost zero to a good level, as you know. Personally, I am very thankful and I wish to see you again.

Jean Bosco SHIMIRWA

It has been a pleasurable and unforgettable experience: learning NGS by performing sequencing using ONT and Illumina as well as analysing and curating the generated data using bioinformatics tools.

Jackson UWIMANA

At the beginning of the training, I was confused about what it would add to my skill box. However, after a few days, I realized that it was all I needed. I liked how collaborative, friendly and professional the trainers were. The training was fun, yet fruitful. It was unforgettable.

Patrick INEZA

I gained much from the training on NGS. The practical part would have been better if the team were small, but I enjoyed it. Thank you very much for the great work you did.

Us Bertin

This training equipped me with bioinformatics command lines and useful pipelines to analyse SARS-CoV-2 genome data. I am now able to analyse SARS-CoV-2 genomic data from both Oxford Nanopore Technology and Illumina platforms. I can apply such knowledge to other pathogens.

Enatha Mukantwari